There are more than 30 different blood groups associated with the human species; understanding the defining genetics of each has been of fundamental importance to medical and healthcare research. Whether it鈥檚 assessing the compatibility of a blood donor for a particular transfusion, or testing for conditions such as Down鈥檚 syndrome in unborn babies, the practical application of that understanding is felt on a daily basis in hospitals across the country.
For Neil Avent, Professor of Transfusion Medicine and Molecular diagnostics, it鈥檚 the very life-blood of his research and has been for more than 25 years. From foundational work on the Rhesus system, one of the most complex and important blood groups, to the development of clinical methods around non-invasive prenatal diagnoses, it鈥檚 research that has spanned the 鈥榖ench to bedside鈥 spectrum.
鈥淚鈥檓 a blood grouper; I look at the molecular basis of blood,鈥 said Neil, Head of the School of Biomedical and Healthcare Sciences. 鈥淏ut from the laboratory to the clinical environment, this knowledge has fundamental and far-reaching impacts and applications, from defining a baby鈥檚 blood group and sex before birth, to assessing the risk that they might have Down鈥檚 syndrome.鈥
Neil鈥檚 early-year research involved developing methods to prenatally define Rhesus D (RhD) status in the clinical management of HDFN (Haemolytic Disease of the Fetus and Newborn). This is the condition where the mother鈥檚 immune system attacks the fetus if they are of opposite Rhesus types (positive or negative), and the mother has previously been exposed to RhD positive blood and has developed an immune response to it. The work contributed directly to the development of non-invasive methods to test for RhD, and the first clinical service in 2001, and was then extended with European funding through the Special Non-invasive Fetal and Neonatal Evaluation, of which Neil was chair.
At Plymouth, Neil and his team work closely with the NHS in the South West, and focus upon two major strands: non-invasive prenatal testing/diagnosis (NIPD) and blood group genotyping. Using an array of sophisticated equipment, including a mass spectrometer called Orbitrap Velos, a digital polymerase chain reaction (PCR) machine BioRad QX100, and a genome sequencer called Ion Torrent, the team has the ability to conduct a wide array of molecular and genetic experiments.
In the field of NIPD, the work has sought to improve prenatal diagnoses of several conditions, reducing the need for invasive procedures such as the amniocentesis, which has a miscarriage rate of 1 per cent and can increase the risk of RhD Haemolytic disease of the fetus and newborn.
Neil said: 鈥淎round 96 per cent of parents want to know if there鈥檚 a Down鈥檚 risk, so what we鈥檙e trying to achieve is a perfectly safe test for them, For the past 10 years we鈥檝e been able to establish sex and blood group of the fetus from maternal blood, but now it is possible to establish the Down鈥檚 risk as well. Anything we can do to reduce the risk to both baby and mother has to be a positive step.鈥
What may be even more ground-breaking is a potential breakthrough in Down鈥檚 testing emanating from the field of proteomics. Thanks to their work with Orbitrap, and research undertaken with collaborators in London, the team believe they may have identified a 鈥榩rotein marker鈥 related to Down鈥檚. If proven correct, it could ultimately lead to the production of a fully diagnostic test, and one which would be much cheaper to administer than current DNA-based ones.
The work surrounding blood group genotyping has been no less significant, with the legacy of Neil鈥檚 15-year involvement evidenced by the commercial product BLOODChip. Used to manage those patients who depend upon blood transfusions to live 鈥 such as those with sickle cell disease 鈥 BLOODchip鈥檚 ability to establish the genetic basis of donor samples helps them to match to those in need of transfusion, thus reducing the risk of complications.
Neil has continued to lead on blood grouping methodology, working with Sanquin in Amsterdam to develop a new technique known as multiplex ligation dependent probe amplification (MLPA) which can analyse thousands of regions of the genome at once, like a molecular abacus, counting chromosomes and identifying irregularities. He鈥檚 also at the forefront of research on next generation sequencing (NGS), which is expected to replace MLPA and PCR methods for blood group genotyping in the near-future, and could pave the way for all blood donors to be genotyped.
鈥淚t鈥檚 all about getting ahead of the game; it鈥檚 looking at new technologies and applying them so that we can move forward to a more personalised form of healthcare and medicine,鈥 Neil added.
